A simple strategy and highly sensitive electrochemical method was developed and described in Britton _ Robinson buffer for Isoniazid determination by Differential Pulse Polarography (DPP). A dropping mercury electrode (DME) was used to characterize the performance of the sensor. The reduction process on the (DME) gave a rise with one peak over
Ep-1.17 v (vs. Ag/AgCl/), within the studied pH range (2 – 9), 3M KNO3 as a supporting electrolyte and 4mm3 mercury drop size at 25°C. A standard calibration plot was conducted in the range between 0.05- 0.40μg.ml-1. The monitored differential Pulse current was directly proportional to the concentration of Isoniazid and it showed a linear response in the studied range (correlation coefficient (r) = 0.9997) and the detection limit
was 0.05μg. ml-1. This method effectively applied in commercial Isoniazid pharmaceuticals.
Publication Date
10/31/2016
Publisher
International Journal of Chemical Engineering Research
سلام عباس حسن | Salam A.H. Al-Ameri | 4755