In this study, the fabrication of tin oxide thin film using pulsed laser deposition technique is reported in detail. Further, the effect of 60Co gamma radiation on the structural, morphological, and optical features is systematically demonstrated using x-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and ultraviolet visible light analysis (UV-Vis). Particularly, the attained crystallite size was found to be decreased from 45.5 to 40.8 nm for pristine and sample irradiated using 1200 Gy 60Co gamma source, respectively. The particle diameter, using FESEM analysis, revealed similar trend to that attained using XRD; in particular, the average diameters were perceived as 93.8 and 79.9 nm for the aforementioned samples. Contrariwise, the calculated optical band gap demonstrated a decreasing profile where optical band gaps of 3.08 and 3.24 eV were acquired for pristine and sample irradiated with 1200 Gy.