The synthesis of hyaluronic acid (HA) by bacteria is a promising alternative to extracting the biopolymer
from animal tissues. The production of HA by Streptococcus thermophilus, which belongs to the group of
organisms generally recognized as safe (GRAS), is a well-studied viable alternative. The present study
investigated five S. thermophilus strains (St1, St2, St3, St4, and St5) with markedly high HA productivity.
The quantity of purified HA was different following the order St5 > St4 > St3 > St2 > St1. The characteristics
of molecular mass analysis of HAs were evaluated by multi-technique (FTIR, NMR, and HPLC). The
antioxidant activity of the purified HA was quantified by multi-technique (DPPH, iron chelating, reducing
power, and hydrogen peroxide (H2O2) scavenging activity), over a range of relevant concentrations, i.e.,
50, 100, 300, 500, 700, 1100, and 1300 mg/ml. They were tested for a concentration-dependent enhancement
in their radical scavenging and lowering abilities. Significantly increased antioxidant activity was
detected at concentrations of 1300 mg/ml, with 69.18%, 78.42%, and 73.74% of DPPH, hydrogen peroxide,
and iron-chelating scavenging activities, respectively. Furthermore, HA was proven to have effective lowering
power at the same concentration. When compared to the typical antioxidant Butylated hydroxytoluene
(BHT), but these various antioxidant activities were low. It is suggested that HA have the
potential to be the resource of natural antioxidant.
Publication Date
4/14/2021
Publisher
Materials Today: Proceedings
Volume No
Issue No
DOI
https://doi.org/10.1016/j.matpr.2021.12.038
Keywords
Hyaluronic acid Antioxidant activity Streptococcus thermophilus. local isolates Acid extracted
علاء كريم نعيمة | Alaa Kareem Niamah | 5685